LB1022 Complete regeneration of secretory glands in salamander skin via blastema-independent mechanism

While human and other mammals are limited in their abilities to regenerate, amphibians, including salamander species such as the axolotl (Ambystoma mexicanum), highly regenerative. Contrary prevailing notion that healing via embryonic-like regeneration necessarily requires a specialized “blastema” tissue, we show axolotls regenerate new skin with anatomically complex secretory glands without blastema that, fact, formation of represses gland-bearing regeneration. We studied regenerative excisional wounds live confocal imaging by which same consecutively re-imaged over span several weeks. This enabled us record entire process gland neogenesis at single-cell resolution fibroblasts closely associate epithelial primordia already onset then profiled regenerating RNA-sequencing (scRNA-seq), is enabling identify key mesenchymal cell populations engaged neogenesis. In mammals, laboratory mice, hair follicles can following large wounding. However, this phenomenon, termed wound-induced (WIHN) typically restricted center wound, wound edges repair hairless scar. Unlike WIHN more complete, occurring across area. Our study on will establish blastema-independent mechanism for likely illuminate understandings broader mechanisms.